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水产养殖科学系

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李强

2023年10月20日 09:58  点击:[]

一、个人简介

山东淄博人,1979年生,教授、博导,天津农学院水生动物医学专业负责人,兼任中国海洋湖沼学会棘皮动物分会理事、中国水产学会水产动物免疫学专业委员会委员。第九届辽宁青年科技奖获得者,曾入选江苏省“333高层次人才培养工程”中青年学术带头人,辽宁省“百千万人才工程”千人层次、辽宁省农业领域青年科技创新人才、辽宁省高等学校杰出青年学者、大连市青年科技之星、盐城市“515”领军人才、辽宁省优秀硕士学位论文指导教师等荣誉称号。

二、研究方向

水产动物疾病快速诊断及绿色防控

比较免疫学

三、代表性科研成果

主持科研项目:

[1] 国家自然科学基金面上项目,波里氏囊在刺参应答病原菌侵染中的作用及机制研究,42076112,58万,202101-202412,主持

[2] 国家自然科学基金面上项目,波里氏囊在刺参排脏后体腔细胞恢复中的作用研究,31872544,59万,201901-202212,主持

[3] 国家自然科学青年基金,30800853,19万,仿刺参"化皮病"病原菌侵染机制的研究,200901-201112,主持

[4] 国家星火计划重点项目,基于微生物调控和免疫防控策略的刺参疾病防控体系示范,2015GA650006,60万,201501-201712,主持

[5] 国家海洋局公益性行业专项子任务,海水养殖动物重要病原库构建及快速检测产品研发,201405003-1,318万,201401-201712,主持

[6] 江苏省重点研发计划(现代农业),安全高效鲫造血器官坏死病弱毒疫苗的研制及应用,BE2021326,100万,202107-202406,主持

[7] 江苏省农业科技自主创新专项,基于胶体金免疫层析技术的异育银鲫鳃出血病快速诊断试纸条研发与应用,CX(18)3028,50万,201808-202108,主持

[8] 辽宁省新型农业社会化服务体系建设示范试点项目,养殖刺参病害快速诊断及综合防治技术,197万,201510-201710,主持

授权国家发明专利:

[1] 国家发明专利,胶体金层析试纸条金标垫的处理方法,授权日2018.4.17,授权号:ZL 201610306907.X,第一发明人。

[2] 国家发明专利,鮰爱德华菌胶体金免疫层析试纸条及制备方法,授权日:2015.2.18,授权号:ZL201310218944.1

[3] 国家发明专利,黄海希瓦氏菌胶体金免疫层析试纸条及制备方法,授权日:2015.4.15,授权号:ZL 201310218943.7

[4] 国家发明专利,灿烂弧菌胶体金免疫层析试纸条及制备方法,授权日:2019.4.2,授权号:ZL 201710795942.7

出版著作和教材:

[1] 江苏省沿海滩涂生物调查与环境评价,上海科学技术出版社,2021(副主编),ISBN 9787547851869.

[2] 动物种业科技创新战略研究报告,科学出版社,2015(副主编),ISBN 9787030442291

[3] 生物质能源产业科技创新发展战略,化学工业出版社,2014(参编),ISBN 9787122191250

[4] 光镜技术及其在水生生物上的应用,中国农业科学技术出版社,2012(参编),ISBN 9787511610454

[5] 2011年执业兽医资格考试应试指南(水生动物类),中国农业出版社,2011(参编)ISBN 9787109177086

[6] 2012年执业兽医资格考试应试指南(水生动物类),中国农业出版社,2012(副主编)ISBN 9787109167421

[7] 2013年执业兽医资格考试应试指南(水生动物类),中国农业出版社,2013(副主编)ISBN 9787109177086

四、代表性论文

[1] Yuan Ren, Yongping Xu, Xuyuan Fan, Bila Murtaza, Yinan Wang, Zhen Li, Muhammad Tariq Javed, Zhenhui Wang*, Qiang Li*. Transcriptome analysis reveals key transcription factors and pathways of polian vesicle associated with cell proliferation in Vibrio splendidus-challenged Apostichopus japonicus. Comparative Biochemistry and Physiology Part D: Genomics & Proteomics, 2023, 46, 101082.

[2] Zhenhui Wang, Xuyuan Fan, Zhen Li, Liyuan Guo, Yuan Ren, Qiang Li*. Comparative analysis for immune response of coelomic fluid from coelom and polian vesicle in Apostichopus japonicusto Vibrio splendidusinfection. Fish and Shellfish Immunology Reports, 2023, 4: 100074.

[3] Jialin Zhang, Shanling Sun, Yiyang Mao, Guo Qiao, Qiang Li*. Identification and analysis of differentially expressed microRNAs in gibel carp Carassius auratus gibelioresponding to polyinosinic-polycytidylic acid (poly IC) stimulation. Fish and Shellfish Immunology Reports, 2023, 4: 100083.

[4] Chang Wei, Chaonan Xu, Yuyu Sun, Jinzhou Li, Motihiko Sano, Qiang Li*. Investigation of the latency of Cyprinid herpesvirus 2 in apparently healthy farmed gibel carp, Carassius auratus gibelio. Aquaculture, 2023,562: 738854.

[5] Zhen Li, Jiajia Hua, Dayu Zhou, Jiayi Feng, Kun Zhou, Qiang Li*. Genomic analysis of a novel Aeromonas veroniiphage pAEv1810, belonging to the genus Petsuvirus. Archives of Microbiology, 2022, 204: 304.

[6] Zhen Li, Jiayi Feng, Guilai Jiang, Dayu Zhou, Jiajia Hua, Qiang Li*.Characterization and genomic analysis of the novel Aeromonas veronii phage pAEv1812. Archives of Virology, 2022, 167:1865-1870.

[7] Jialin Zhang#, Shaodong Meng#, Heng Ge, Shanling Sun, Xinran Yu, Guo Qiao, Qiang Li*.Comparison of viral replication, antiviral gene expression and antioxidant enzyme activity in gibel carp (Carassius auratus gibelio) infected with cyprinid herpesvirus2 at susceptible and less-susceptible temperatures.Turkish Journal of Fisheries and Aquatic Sciences, 2022,22(4): TRJFAS20623.

[8] Guo Baoqin, Wei Chang, Luan Linlin, Zhang Jialin, Wang Yinan, Li Qiang*. Production and application of monoclonal antibodies against ORF66 of cyprinid herpesvirus2. Journal of Virological methods, 2022, 299: 114342.

[9] Guo Liyuan#, Wang Zhenhui#, Shi Weibo, Wang Yinan, Li Qiang*. Transcriptome analysis reveals roles of polian vesicle in sea cucumber Apostichopus japonicusresponse to Vibrio splendidus. Comparative Biochemistry and Physiology Part D: Genomics & Proteomics, 2021, 40, 100877.

[10] Zhang Jialin, Cui Zhengyi, Hu Guangyao, Jiang Xinyu, Wang Jia, Qiao Guo, Li Qiang*. Transcriptome analysis provides insights into the antiviral response in the spleen of gibel carp (Carassius auratus gibelio) after poly I: C treatment. Fish & Shellfish Immunology, 2020,102:13-19 .

[11] Qiao Guo,Lv Tingli,Zhang Mingming, Chen Peng, Sun Qirui, Zhang Jialin, Li Qiang*.β-hydroxybutyrate (β-HB) exerts anti-inflammatory and antioxidant effects in lipopolysaccharide (LPS)-stimulated macrophages in Liza haematocheila. Fish & Shellfish Immunology, 2020,107:444-451 .

[12] Zhang Mingming, Wang Jiawei, Sun Qirui, Zhang Hu, Chen Peng, Li Qiang*, Wang Yinan, Qiao Guo*.Immune response of molluscs Onchidium strumato extremely low-frequency electromagnetic fields (ELF-EMF, 50 Hz) exposure based on immune-related enzyme activity and De novo transcriptome analysis. Fish & Shellfish Immunology,2020, 98: 574-584.

[13] Ren Yuan#, Zhang Jialin#, Wang Yinan, Chen jing, Liang Chunlei, Li Ruijun, Li Qiang*. Non-specific immune factors differences in coelomic fluid from polian vesicle and coelom of Apostichopus japonicus, and their early response after evisceration. Fish & Shellfish Immunology2020, 98:160-166.

[14] Qiao Guo#,Chen Peng#, Sun Qirui, Zhang Mingming, Zhang Jialin, Li Zhen, Li Qiang*. poly-β-hydroxybutyrate (PHB) in bioflflocs alters intestinal microbial community structure, immune-related gene expression and early Cyprinid herpesvirus2 replication in gibel carp (Carassius auratus gibelio). Fish & Shellfish Immunology2020, 97: 72-82.

[15] Shi Weibo#, Zhang Jialin#, Wang Yinan, Ji Jinlin, Guo Liyuan, Ren Yuan, Qiao Guo, Wang QingKui, Li Qiang*. Transcriptome analysis of Apostichopus japonicus polian vesicles in response to evisceration. Fish & Shellfish Immunology, 2020, 97:108-113.

[16] Zhang Mingming#, He Pei#,Qiao Guo, Wang Jiawei, Huang Jintian, Yuan Xiutang, Li Qiang*. Distribution, sources, and risk assessment of polycyclic aromatic hydrocarbons (PAHs) in surface sediments of the Subei Shoal, China. Marine Pollution Bulletin, 2019, 149:110640.

[17] Qiao Guo, Sun Qirui, Zhang Mingming, Xu Chen, Lv Tingli, Qi Zhitao, Yang Wenping,Li Qiang* Antioxidant system of soiny mullet (Liza haematocheila) is responsive to dietary poly-β-hydroxybutyrate (PHB) supplementation based on immune-related enzyme activity and de novo transcriptome analysis. Fish & Shellfish Immunology, 2019, 95:314-327.

[18] Qiao Guo#, Xu Chen#, Sun Qirui, Xu Dehai, Zhang Mingming, Chen Peng, Li Qiang*.Effects of dietary poly-β-hydroxybutyrate supplementation on the growth, immune response and intestinal microbiota of soiny mullet (Liza haematocheila).Fish & Shellfish Immunology, 2019, 91:251-263.

[19] Zhang Mingming#, He Pei#, Qiao Guo, Huang Jintian, Yuan Xiutang, Li Qiang*. Heavy metal contamination assessment of surface sediments of the Subei Shoal,China: Spatial distribution, source apportionment and ecological risk. Chemosphere, 2019,223:211-222.

[20] Li Qiang#, Ren Yuan#, Luan Linlin, Zhang Jialin, Qiao Guo, Wang Yinan, Ye Shigen, Li Ruijun.Localization and characterization of hematopoietic tissues in adult sea cucumber, Apostichopus japonicus. Fish & Shellfish Immunology, 2019, 84:1-7.

[21] Li Qiang, Qiao Guo, Wang Li, Zhang Jipeng, Li Ruijun, Ni Ping, Guo Yi, Ye Shigen. Studies on Bacterial Diversity and Vibrio harveyi Distribution Associated with Diseased fugu (Takifugu rubripes) in Northeastern China. Pakistan Journal of Zoology, 2019, 51(1):67-77.

[22] Zhang Mingming, Xu Yang, Kim Sukyoung, Wu Qiuxian, Qi Zhitao, Pang Zhenguo, Qiao Guo*, Li Qiang*. Molecular Characterization, Structure and Expression Analysis of A Ras-related Nuclear (Ran) Gene in Pacific White Shrimp,Litopenaeus vannamei. International Journal of Agriculture and Biology, 2019, 21(1): 11-18.

[23] Zhang Mingming, He Pei, Liu Pu, Shen Yonglong, Qiao Guo*, Li Qiang*, Huang Jintian. Acute toxicity of heavy metals toOnchidium strumaunder different salinities. International Journal of Agriculture and Biology, 2019, 21:307-313.

[24] Li Qiang#, Ren Yuan#, Liang Chunlei, Qiao Guo, Wang Yinan, Ye Shigen, Li Ruijun. Regeneration of coelomocytes after evisceration in the sea cucumber, Apostichopus japonicus. Fish & Shellfish Immunology, 2018, 76:266-271.

[25] Wang Yinan, Meng Shaodong, Zhang Jialin, Ding Jun, Li Qiang*. Production, characterization and application of monoclonal antibodies to the coelomocytes of sea urchin Strongylocentrotus Intermedius.Fish & Shellfish Immunology, 2018, 75:301-307.

[26] Qiao Guo, Zhang Mingming, Li Ye, Xu Chen, Xu Dehai, Zhao Zhigang, Zhang Jialin, Li Qiang*. Biofloc technology (BFT): An alternative aquaculture system for prevention of Cyprinid herpesvirus2 infection in gibel carp (Carassius auratus gibelio).Fish & Shellfish Immunology, 2018, 83:140-147.

[27] Ye Shigen, Xie Mingxu, Li Ruijun, Zhang Chao, Yang Jing, Bai Lu, Li Qiang*.Screening and Characterization of Acid-Producing Bacteria from High pH Saline-alkali Areas. International Journal of Agriculture and Biology, 2018, 20(7):1682-1690.

[28] Ye Shigen, Liu Meiru,Wang Li, Li Ruijun, Li Qiang*. Composition and Diversity of Intestinal Microbiota of Sea Cucumber Apostichopus japonicusfrom Different Habitats. International Journal of Agriculture and Biology, 2018,20(8):1868-1872.

[29] Li Ruijun, Gao Yanqi, Hou Yulin, Ye Shigen, Wang Lianshun, Sun Jingxian, Li Qiang*. Mitochondrial genome sequencing and analysis of scuticociliates Uronema marinum isolated from Takifugu rubripes.Mitochondrial DNA Part B, 2018, 3(2), 736-737.

[30] Li Ruijun, Zhang Xianyu, Yang Chuan, Ye Shigen, Li Hua,Li Qiang*. Development of a Colloidal Gold-Based Immunochromatographic Assay for the Rapid Detection ofEdwardsiella Ictaluri. Journal of Nanoscience and Nanotechnology, 2018, 18(6): 3800-3805.

[31] Zhang Mingming, Li Ye, Xu Dehai, Qiao Guo*, Zhang Jialin, Qi Zhitao, Li Qiang*.. Effect of different water biofloc contents on the growth and immune response of gibel carp cultured in zero water exchange and no feed addition system. Aquaculture research, 2018,49:1547-1556.

[32] Li Qiang, Wang Youjuan, Zhang Mingming,Li Ruijun,Ye Shigen, Li Hua*.Production and characterization of monoclonal antibodies against recombinant ORF 049L of rock bream iridovirus. Process Biochemistry, 2016,51(12):2238-2242.

[33] Li Qiang, Qi Rui-rong, Wang Yi-nan, Li Hua*Ontogenesis of coelomocytes in sea cucumber (Apostichopus japonicus) studied with probes of monoclonal antibody. Fish & Shellfish Immunology, 2014, 41(2):260-263.

[34] Li Qiang, Qi Rui-rong, Wang Yi-nan, Ye Shi-gen, Qiao Guo, Li Hua*. Comparison of cells free in coelomic and water-vascular system of sea cucumber Apostichopus japonicus. Fish & Shellfish Immunology, 2013, 35:1654-1657.

[35] Li Qiang, Jing Hong-li, Li Hua*, Wang Yi-nan, Xu De-hai. Characterization and application of monoclonal antibodies against Shewanella marisflavi, a novel pathogen of Apostichopus japonicus. Chinese journal of Oceanology and Limnology, 2011, 29(5):973-980.

[36] Li Qiang, Li Ying, Li Hua*, Wang Yinan, Xu De-hai. Production characterization and application of monoclonal antibody to spherulocytes A subpopulation of coelomocytes ofApostichopus japonicus. Fish & Shellfish Immunology, 2010, 29:832-838.

[37] Li Qiang, Zhan Wen-bin*, Xing Jing, Sheng Xiu-zhen. Production, characterisation and applicability of monoclonal antibodies to immunoglobulin of Japanese flounder (Paralichthys olivaceus). Fish & Shellfish Immunology, 2007, 23:982-990.

[38] Li Ruijun, Huang Hua, Zhang Xianyu, Ye Shigen, Li Qiang*. Monoclonal antibody based Dot-ELISA and indirect fluorescence antibody technique for detecting Edwardsiella ictaluriinfection in yellow catfish (Pelteobagrus fulvidraco). Aquaculture and Fisheries, 2017, 2:207-212.

[39] 范栩源,王振辉,任媛,国丽媛,孙启睿,王轶南,李强*.刺参水管系统与体腔间物质交换问题探究. 水产科学,2023,42(4):705-711.

[40] 史伟卜,王轶南,王浩文,任媛,王庆奎,李强*.转录组测序解析刺参波里氏囊腔与体腔中体腔细胞对吐脏胁迫的响应差异.海洋学报,2021,43(2):116-125.

[41] 郭宝琴,魏畅,李强*.鲤疱疹病毒Ⅱ型检测技术的研究进展,水产科学,2021,40(6):943-950.

[42] 任媛,李强*,王轶南,李振. 棘皮动物体腔细胞的研究进展. 中国农业科技导报,2019,21(2):91-97.

[43] 于新然,姚洪,叶仕根,黎睿君,李华,李强*. 养殖大菱鲆感染迟缓爱德华氏菌的分离、毒力基因及 ERIC-PCR分型. 大连海洋大学学报,2018,33(2):169-174.

[44] 徐阳,姚洪,乔帼,李华,叶仕根,黎睿君,李强*. 养殖大菱鲆感染嗜水气单胞菌的分离、毒力基因及 ERIC-PCR分型. 大连海洋大学学报,2018,33(4):1-5.

[45] 王博雅,王力,刘美如,叶仕根,黎睿君,李华,李强*. 凡纳滨对虾3种主要病毒和虾肝肠胞虫在辽宁地区的流行情况分析. 大连海洋大学学报,2017,32(2):150-154.

[46] 姚刚,李强*,付雷,叶仕根,黎睿君,李华. 几种中草药对刺参幼参的急性毒性试验. 大连海洋大学学报,2017,32(2):161-166.

[47] 杨川,秦艺丹,胡潜,李强*. 抗迟缓爱德华菌单克隆抗体的制备及双抗夹心ELISA检测方法的建立. 生物技术通报,2016,32(7):200-205.

[48] 郑斌,郝振林,王轶南,刘远,李强*,李向宇,陈庭军. 海蜇养殖池塘底质环境对其产量影响的研究. 大连大学学报,2016,37(3):65-70.

[49] 秦艺丹,李男,李洋,李强*. 抗红鳍东方鲀皮肤溃烂病病原哈维弧菌单克隆抗体的制备及特性分析. 现代农业科技,2016(8):239,260.

[50] 郑斌,邹绍林,姚洪,解明旭,杨晓宇,李强*,李华. 池塘养殖海蜇疾病的病因分析. 中国水产,2016(4):82-86.

[51] 郝振林,郑斌,李强*,李向宇,陈庭军. 环境条件对海蜇生长发育影响的研究进展. 大连海洋大学学报,2015,30(4):444-448.

[52] 张家林,李强*,叶仕根,李华. 鲤春病毒血症病毒糖蛋白的原核表达及单克隆抗体制备. 大连海洋大学学报,2014,29(5):454-458.

[53] 张家林,李洋,李强*. 鲤春病毒血症病毒G蛋白的研究进展. 生物技术通报,2014(5):21-25.

[54] 李强,黄华,张显昱,叶仕根,李华. 鮰爱德华菌单克隆抗体的制备及在黄颡鱼“红头病”研究中的应用. 大连海洋大学学报,2014,29(4):323-328

[55] 胡潜,李强*,张显昱.传染性造血器官坏死病毒(IHNV)研究现状. 现代农业科技,2014(7):273-277.

[56] 李洋,李强*,张显昱. 哈维弧菌及其主要致病因子的研究进展. 中国农业科技导报,2014,16(4):159-166.

[57] 李强,孙康泰,张显昱. 刺参“ 腐皮综合征” 研究进展. 中国农业科技导报,2013,15(6):40-45.

[58] 张显昱,刘志强,李强*. 胶体金免疫层析试纸条在水产养殖业中的应用. 生物技术通报,2013,12:57-62.

[59] 张显昱,李强*,李明,黄华,叶仕根,李华. 两种鮰爱德华菌ELISA快速检测方法的建立与应用. 中国农业科技导报,2013,15(3):175-182.

[60] 黄华,杜天舒,李强*,王轶南,李华. 单克隆抗体在水产病原微生物检测中的应用. 中国农业科技导报,2012,14(2),145-150.

[61] 戚瑞荣,李强*,于毅,李华. 单克隆抗体在水产无脊椎动物血淋巴细胞研究中的应用. 中国农业科技导报,2012,14(1):146-151.

[62] 于洪,戚瑞荣,李强*,李华. 对仿刺参排脏后体腔细胞恢复过程的研究. 大连海洋大学学报,2012,27(增刊):1-4.

[63] 李强,齐楠,杨丽文,李华,王轶南. 黄海希瓦氏茵多克隆抗体部分特性的分析. 大连海洋大学学报,2011,26(4):376-380.

[64] 李强,刘海燕,黄华,李华. 鮰爱德华菌黄颡鱼分离株外膜蛋白的抗原性分析. 广东海洋大学学报,2011,31(3):85-90.

[65] 吴秋仙,李强*,李华,王轶南. 应用双抗体夹心酶联免疫方法检测仿刺参病原茵—黄海希瓦氏菌AP629. 中国农业科技导报,2011,13(1):117-121.

[66] 李强,迟刚,李莹,李华. 仿刺参体腔细胞单克隆抗体的制备及特性分析. 大连海洋大学学报,2010,25(6):542-546.

[67] 景宏丽,李强*,吴秋仙,李华. 黄海希瓦氏菌单抗介导间接ELISA快速检测技术的建立. 大连海洋大学学报,2010,25(6):547-550.

[68] 李重实,李强*,刘海燕,叶仕根,李华. 鲶爱德华氏菌间接酶联免疫快速检测法的建立. 广东海洋大学学报,2010,30(4):17-21.

[69] 李强,乔帼,付雷,李华. 凡纳滨对虾感染白斑综合症病毒后的新症状. 水产科学,2008,27(9):465-469.

[70] 李强,李华,姜传俊,王吉桥. 温度对凡纳滨对虾血淋巴免疫指标的影响. 大连水产学院学报,2008,23(2):132-135.

[71] 李强,战文斌,绳秀珍,邢婧,周丽. 应用单克隆抗体检测牙鲆血清中抗淋巴囊肿病毒的特异性抗体. 中国海洋大学学报,2007,37(2):243-246.

[72] 李强,罗永成,李华,常亚青. 常用抗茵药物和消毒剂对刺参幼体的急性毒性试验. 大连水产学院学报,2005,20(2):105-110.

五、获奖情况

[1] 新型海水养殖疾病防治绿色系列产品研发及其产业化应用,大连市科技进步一等奖,第五完成人2021年2月,证书号2020J-1-01-R05.

[2] 刺参疾病的发生机制及无公害防控技术研究与应用,辽宁省科技进步二等奖,第二完成人2014年11月,证书号2014J-2-15-02.

[3] 刺参疾病无公害防控技术的研究与应用,大连市科技进步一等奖,第二完成人2014年11月,证书号2013-J-01-02.

[4] 刺参疾病的发生机制及无公害防控技术研究与示范,国家海洋局海洋科学技术二等奖,第二完成人2014年5月,证书号HKJ2013-G-2-B32-02.

[5] 鱼类淋巴囊肿病毒单克隆抗体研制及检测诊断技术与流行病学研究.农业部中华农业科技三等奖,第六完成人2009年12月,证书号KJ2009-G3-044-06.

[6] 鱼类淋巴囊肿病毒中和单克隆抗体研制及其流行病学研究.国家海洋局海洋创新成果二等奖,第六完成人2007年11月,证书号200722306B.

[7] 海水鱼类淋巴囊肿病毒病的研究,教育部自然科学二等奖,第六完成人2008年1月,证书号2007-089.

[8] 鲤鱼高抗新品种选育关键技术研究及应用,黑龙江省农业科学技术一等奖,第四完成人2015年2月,证书号2014-01-09-04.

[9] 刺参体腔液球形细胞单克隆抗体的制备、特性分析及应用,辽宁省自然科学学术成果一等奖(学术论文类),第一完成人2011年7月,2011-LNL1855.

六、联系方式

邮箱:fish790608@163.com

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